Blocking Assay:Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4° C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit anti-GluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, #AB190289, 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 hours at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty.
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit antiGluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, #AB190289, 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 h at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit anti-GluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, # AB190289 , 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 h at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: A single cell transcriptional roadmap of human pacemaker cell differentiation
Article Snippet: .. Permeabilization was performed with 0.1% Triton- X (Sigma- Aldrich #T8787) and a blocking step was carried out with 4% swine serum (Jackson ImmunoResearch, #014- 000- 121) for 1 hr. ..
Article Title: The VGCC auxiliary subunit α2δ1 is an extracellular GluA1 interactor and regulates LTP, spatial memory, and seizure susceptibility
Article Snippet: .. After blocking tissue with 5% swine serum (Jackson Immuno Research, # 014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), samples were incubated overnight at 4° C with the following primary antibodies: GluA1 (rabbit, Cell signaling, #13185, 1:500 dilution), PSD-95 (mouse, Synaptic Systems, #124 011, 1:500), and VGLUT1 (guinea pig, Synaptic Systems, #135 304, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 1.5 hour at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, # 8961S).
Incubation:Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4° C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit anti-GluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, #AB190289, 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 hours at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty.
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit antiGluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, #AB190289, 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 h at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: The GluA1 cytoplasmic tail regulates intracellular AMPA receptor trafficking and synaptic transmission onto dentate gyrus GABAergic interneurons, gating response to novelty
Article Snippet: .. After blocking with 5% swine serum (Jackson Immuno Research, #014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, #T8787), samples were incubated overnight at 4 °C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems, #182 105, 1:500), rabbit anti-GluA3 (Alomone Labs, #AGC-010, 1:500), rabbit anti-GluA4 (Cell Signaling, #8070, 1:500), rabbit anti c-Fos (Abcam, # AB190289 , 1:500) and mouse anti PSD-95 (Synaptic Systems, #124 011, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 594 goat anti-rabbit (Life Technologies, #A11012, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 2 h at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, #8961S).
Article Title: The VGCC auxiliary subunit α2δ1 is an extracellular GluA1 interactor and regulates LTP, spatial memory, and seizure susceptibility
Article Snippet: .. After blocking tissue with 5% swine serum (Jackson Immuno Research, # 014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), samples were incubated overnight at 4° C with the following primary antibodies: GluA1 (rabbit, Cell signaling, #13185, 1:500 dilution), PSD-95 (mouse, Synaptic Systems, #124 011, 1:500), and VGLUT1 (guinea pig, Synaptic Systems, #135 304, 1:500) followed by incubation with Alexa 488 goat anti-mouse (Life Technologies, #A-11001, 1:500), Alexa 647 goat anti-rabbit (Life Technologies, #A21245, 1:500) and Alexa 568 goat anti-guinea pig (Life Technologies, #A11075, 1:500) secondary antibodies for 1.5 hour at RT. .. Slides were mounted with ProLong Gold Antifade Reagent with DAPI (Cell Signaling Technology, # 8961S).
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